| Role | Voice Part | Classification |
|---|---|---|
| Cléopâtre | Mezzo-Soprano | L |
| Marc-Antoine | Baritone | L |
| Octavie | Soprano | F |
| Spakos | Tenor | F |
| Charmion | Soprano | S |
| Ennius | Baritone | S |
| Amnhès | Baritone | B |
| Sévérus | Baritone | B |
| Slave in Act 2 | Baritone | CB |
| Slave in Act 4 | Baritone | CB |
| A voice | Baritone | CB |
| Minimum Chorus: 32 | ||
| Divisi: SATB |