| Role | Voice Part | Classification |
|---|---|---|
| Daphne | Soprano | L |
| Apollo | Tenor | L |
| Gaea | Contralto | F |
| Leukippos | Tenor | F |
| 1st Maid | Soprano | S |
| 2nd Maid | Soprano | S |
| Peneios | Bass | S |
| 1st Shepherd | Baritone | B |
| 2nd Shepherd | Tenor | B |
| 3rd Shepherd | Bass | B |
| 4th Shepherd | Bass | B |
| Minimum Chorus: 16 | ||
| Divisi: TB |