| Role | Voice Part | Classification |
|---|---|---|
| Elizabeth de Valois, later Queen of Spain | Soprano | L |
| Princess Eboli, her lady-in-waiting | Mezzo-Soprano | L |
| Don Carlos, heir to the throne | Tenor | L |
| Rodrigo, Marquess of Posa | Baritone | L |
| Philip II, King of Spain | Bass | L |
| The Grand Inquisitor | Bass | F |
| A Monk (Friar) | Bass | S |
| Tebaldo, Elizabeth's page | Soprano | S |
| A Heavenly Voice | Soprano | S |
| Count Lerma | Tenor | B |
| The Royal Herald | Tenor | B |
| Forester (in the V Act version of the opera) | Baritone | B |
| Countess D'Aremburg | Mime | |
| Minimum Chorus: 40 | ||
| Divisi: SATB |
Schedule C