| Role | Voice Part | Classification |
|---|---|---|
| Fra Diavolo (Marquis of San Marco) | Tenor | L |
| Zerlina | Soprano | L |
| Lorenzo | Tenor | F |
| Lord Cockburn | Tenor | F |
| Lady Pamela | Mezzo-Soprano | F |
| Giacomo | Bass | F |
| Beppo | Tenor | F |
| Matteo | Bass | S |
| Contadino (Peasant) | Tenor | CB |
| Soldier | Tenor | CB |
| Minimum Chorus: 28 | ||
| Divisi: SATB |