| Role | Voice Part | Classification |
|---|---|---|
| Dejanira | Soprano | L |
| Iole | Soprano | L |
| Lichas | Countertenor | L |
| Hyllus | Tenor | L |
| Hercules | Bass | F |
| Priest of Jupiter | Bass | S |
| An Oechalian Virgin | Soprano | CB |
| Chorus Alto | Alto | CB |
| Chorus Tenor | Tenor | CB |
| A Trachinian | Bass | CB |
| Minimum Chorus: 24 | ||
| Divisi: SATB |