| Role | Voice Part | Classification |
|---|---|---|
| Jenůfa | Soprano | L |
| Laca Klemeň | Tenor | L |
| Kostelnička Buryjova | Soprano | L |
| Števa Buryja | Tenor | F |
| Grandmother Buryja | Contralto | S |
| Foreman at the Mill (Old Mate) | Baritone | S |
| Mayor of the Village | Bass | B |
| Karolka, his daughter | Mezzo-Soprano | B |
| Barena (Servant at the Mill) | Soprano | B |
| The Mayor's Wife | Mezzo-Soprano | B |
| The Maid (Old Shepherdess) | Mezzo-Soprano | B |
| Jano (Shepherd Boy) | Soprano | B |
| Aunt | Contralto | CB |
| A Woman's Voice | Soprano | CB |
| A Man's Voice | Baritone | CB |
| Minimum Chorus: 26 | ||
| Divisi: SATB |
Schedule C