| Role | Voice Part | Classification |
|---|---|---|
| Don Alfonso (Duke of Ferrara) | Baritone | L |
| Lucrezia Borgia | Soprano | L |
| Gennaro | Tenor | L |
| Maffio Orsini | Mezzo-Soprano | L |
| Rustighello | Tenor | S |
| Jeppo Liverotto | Tenor | B |
| Oloferno Vitellozo | Tenor | B |
| Don Apostolo Gazella | Bass | B |
| Ascario Petrucci | Bass | B |
| Gubetta | Bass | B |
| Astolfo | Bass | B |
| Usciere | Tenor | CB |
| Coppiere | Bass | CB |
| Le Dame (Ladies - Act II) | Soprano & Mezzo-Soprano | CB |
| A Voice (Off-Stage - Act II) | Bass | CB |
| Principessa Negroni (Act II) | Mute | CB |
| Minimum Chorus: 24 | ||
| Divisi: SATB |
Schedule C