| Role | Voice Part | Classification |
|---|---|---|
| Elizabeth I | Soprano | L |
| William Shakespeare | Tenor | L |
| Sir John Falstaff | Baritone | L |
| Olivia | Mezzo-Soprano | F |
| Lord Latimer | Tenor | F |
| Jérémy | Bass-Baritone | S |
| Nelly | Soprano | B |
| Lady in Waiting | Spoken | CB |
| Usher | Spoken | CB |
| Minimum Chorus: 24 | ||
| Divisi: SATB |